Applicability of a new cell culture device (Petaka G3™) for cooled-stored of stallion semen
The study suggests that equine semen can be stored in Petaka G3™ cell cul-ture devices, without negatively affecting quality of spermatozoa. Correlating the values obtained for every parameter tested with the relatively low price Petaka G3™ cell cul-ture devices can be considered an alternative to traditional methods for preserving cooled-stored stallion semen.
September 2010: Life Science Research Edition
featuring Amresco Regeneration columns for DNA purification, SapphireAmp Fast PCR Master Mix, Screentape system for automated electrophoresis and eco-friendly disposables.
Investigator: Volume 27, Number 1, 2011
featuring New products: EZ-Vision® In-Gel Solution, Protein EZ-Vision® , EZ-Vision® MW Ladders and EZ-Vision® DNA Dye as Loading Buffer and RNA EZ-Vision® Dye as Loading Buffer.
March 2011: Life Science Research Edition
Featuring: EZ Vision; safe Ethidium Bromide alternative, gelelectrophoresis 1 min, Optiprep; isolating of cells, organelles, viruses, macromolecules etc. and the Vapro Osmometer; new model with self cleaning thermocouple.
September 2011 - Complete automated DNA procedure to facilitate DNA database collection
To address the requirements of DNA database workflow, a complete automated procedure is necessary. Analysis by direct PCR on FTA® punches is possible. However, since DNA quantity is highly variable from one sample to another, genotyping STR profiling leads to a very high rate of invalidated genetic profiles (20-25%). In order to be in the best amplification conditions, it is important to isolate good quality DNA in a normalized concentration. To meet this requirement in one step, we used Smart D-N-Adem-kit for profiling (ADEMETCH) containing calibrated magnetic nanoparticles suitable with a fast and completely automated procedure, from DNA extraction to injection plate setup. The DNA extraction and normalization procedure was successfully validated on 2047 FTA® cards, leading to 95.5% success rate with av. PHR of 86.5%. DNA quantification is no longer necessary prior to STR typing. Similar results have been shown with buccal sample swabs.